Description
This assay employs an antibody specific for Anti-human IFN- Alpha coated on a 96-well plate. Standards and samples are pipetted into the wells and IFN- Alpha present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human IFN- Alpha antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IFN- Alpha bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm